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Фізико-технічний інститут
Кафедра Прикладної фізики
<Головна Кафедра Навчання Наука Форум Download Новини Мапа>

Current Position and Address:

  • Professor of Biophysics
  • Leading Scientist and Head of the Laboratory
  • Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Bogomoletz Str., 4, 01024 Kiev-24, Ukraine.
  • Tel./Fax: +380-44-256-2435
  • E-mail: yshuba@biph.kiev.ua

Education and Degrees:

Institution and location Degree Year
  Field of study
  • Kiev State University, Kiev, USSR (1972-1977)
M.S.1977  Radiophysics
  • Kiev affiliate of Moscow Physical and Technical Institute, Kiev, USSR (1980-1983)
Ph.D.1983  Physiology & Biophysics
  • Bogomoletz Institute of Physiology, Kiev, USSR
D.Sc.1991  Physiology & Biophysics
Thesis titles
  • Candidate of Science (Ph.D.) thesis: "The effect of Ca2+-chelating agents on selectivity properties of voltage-gated Ca2+ channels"
  • Doctor of Science (D.Sc., second degree) thesis: "Voltage-gated Ca2+ channels: types, properties, pharmacology and regulation"

Academic Positions:

  • 03/2007-present
Deputy Director, International Center of Molecular Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine.
  • 02/2006-present
Head of the Laboratory of Molecular Biophysics, Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine.
  • 06/1997-present
Deputy Head of the Department of General Physiology of Nervous System, Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine.
  • 07/1993-present
Leading Scientist, Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine.
  • 11/1998-present
Invited Professor (3-4 months per academic year), Laboratory of Cellular Physiology, INSERM EPI-9938/EMI-0228, University of Lille 1, France.
  • 01/1997-02/2007
Research group leader, International Center of Molecular Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine.
  • 09/1993-12/1996
Visiting Assistant Professor of Pharmacology, Department of Pharmacology, Georgetown University, Washington, DC, USA.
  • 08/1991-07/1993
Visiting Scientist, Department of Physiology & Biophysics, Dalhousie University, Halifax, Canada.
  • 06/1983-07/1993
Junior Scientist, Scientist, Senior Scientist, Bogomoletz Institute of Physiology, Ukrainian Academy of Sciences, Kiev, USSR/Ukraine.
  • 10/1988-07/1989
Research Fellow, II Physiological Institute, University of Saarland, Homburg, Germany, (Prof. W. Trautwein).
  • 06/1980-05/1983
Aspirant (graduate student, Ph.D. program) in Physiology & Biophysics, Moscow Physical and Technical Institute, USSR.
  • 09/1977-05/1980
Research Associate in Plasma Physics, Institute of Physics, Ukrainian Academy of Sciences, Kiev, USSR.

Field of expertise:

  • Structure, function and regulation of membrane ion channels, receptors and transporters.

Experimental techniques used:

  • Variety of electrophysiological techniques (patch-clamp, single channel recording, double microelectrode voltage-clamp, intracellular perfusion methods); isolation of single cells from various tissues; cells culturing; isolation, maintenance and injection of Xenopus oocytes.

Grants and Awards:

At different times was supported by grants from:
  • INTAS (International Association for the promotion of co-operation with scientists from the New Independent States of the former Soviet Union);
  • Fogarty International Center;
  • American Heart Association Nation's Capital Affiliate;
  • Georgetown University intramural Clinical-Basic Science grant;
  • International Soros Science Foundation.

Honors:

  • 2003 Ukrainian State Award in the field of science and technology

Memberships:

  • Ukrainian Biophysical Society, USA Biophysical Society

Selected publications:


I. Selected English language reviewed journal articles (arranged by topics)
  • Neuronal Ca2+ channels
Shcheglovitov A., Zhelay T., Vitko Y., Osipenko V., Perez-Reyes E., Kostyuk P. and Shuba Y. Contrasting the effects of nifedipine on subtypes of endogenous and recombinant T-type Ca2+ channels. Biochem Pharmacol. 69(5):841 - 854 (2005).

Subtypes of low voltage-activated Ca2+ channels in the associative thalamic neurons: possible localization and physiological roles. - Zhuravleva S.O., Kostyuk P.G. and Shuba Ya.M. Pflügers Arch. 441:832-839 (2001).

Divalent cation selectivity of the subtypes of low voltage-activated Ca2+ channels in thalamic neurons. - Zhuravleva S.O., Kostyuk P.G. and Shuba Ya.M. NeuroReport 10:651-657 (1999).

Expression of Ca2+ channels from rat brain with model phenylketonuria in Xenopus oocytes. - Dzhura I., Naidenov V., Zhuravleva S., Kostyuk P. and Shuba Y. Brain Res. 783:280-285 (1998).

Characterization of hypothalamic low-voltage-activated Ca channels based on their functional expression in Xenopus oocytes. - Dzhura I.A., Naidenov V., Lyubanova O.P., Kostyuk P.G. and Shuba Ya.M. Neuroscience 70:729-738 (1996).

Expression of low-voltage-activated Ca2+ channels from rat brain neurons in Xenopus oocytes. - Dzhura I.A., Kostyuk P.G., Lyubanova O.P., Naidenov V. and Shuba Ya.M. NeuroReport 5:1960-1962 (1994).

The effect of permeant ions on single calcium channel activation in mouse neuroblastoma cells: ion-channel interaction. - Shuba Ya.M., Teslenko V.I., Savchenko A.N. and Pogorelaya N.H. J. Physiol. 443:25-44 (1991).

Activation kinetics of single high-threshold calcium channels in the membrane of sensory neurons from mouse embryos. - Kostyuk P.G., Shuba Ya.M. and Teslenko V.I. J. Membr. Biol. 110:29-38 (1989).

Three types of calcium channels in the membrane of mouse sensory neurons. - Kostyuk P.G., Shuba Ya.M. and Savchenko A.N. Pflügers Arch. 411:661-669 (1988).

Two ion-selecting filters in the calcium channel of the somatic membrane of mollusc neurons. - Kostyuk P.G., Mironov S.L. and Shuba Ya.M. J. Membr. Biol. 76:83-93 (1983).

  • Cardiac electrophysiology, modulation of cardiac K+ channels
Testosterone-mediated modulation of HERG blockade by proarrhythmic agents. - Shuba Y.M., Degtiar V.E., Osipenko V.N., Naidenov V.G. and Woosley R.L. Biochem. Pharm. 62:41-49 (2001).

A novel molecular determinant for cAMP-dependent regulation of the frog heart Na+-Ca2+ exchanger. - Shuba Y.M., Iwata T., Naidenov V.G., Oz M., Sandberg K., Kraev A., Carafoli E. and Morad M. J. Biol. Chem. 273:18819-18825 (1998).

Beta-adrenoceptor-coupled Gs protein facilitates the activation of cAMP-dependent cardiac Cl- current. - Pelzer S., You Y., Shuba Y.M. and Pelzer D.J. Am. J. Physiol. (Heart Circ. Physiol.) 273:H2539-H2548 (1997).

cAMP-dependent regulation of Ca2+ channels expressed in Xenopus oocytes. - Shuba Y.M., Naidenov V.G. and Morad M. Neurophysiology (Kiev) 29:40-49 (1997).

Comparative effects of loratadine and terfenadine on cardiac K+ channels. - Ducic I., Ko C.M, Shuba Y.M. and Morad M. J. Cardiovasc. Pharm. 30:42-45 (1997).

Suppression of mammalian cardiac K+ channel family by ebastine. - Ko C.M., Ducic I., Fan J., Shuba Y.M. and Morad M. J. Pharm. Exp. Ther. 281:233-244 (1997).

Regulation of sodium-calcium exchanger by beta-adrenergic agonists in frog ventricular myocytes. - Fan J., Shuba Y.M. and Morad M. Proc. Natl. Acad. Sci. USA 93:5527-5532 (1996).

Membrane-delimited stimulation of heart cell calcium current by β-adrenergic signal-transducing Gs protein. - Pelzer S., Shuba Ya.M., Asai T., Codina J., Birnbaumer L., McDonald T.F. and Pelzer D. Am. J. Physiol. (Heart Circ. Physiol.) 259:H264-H267 (1990).

Whole-cell calcium current in guinea-pig ventricular myocytes dialyzed with guanine nucleotides. - Shuba Ya.M., Hesslinger B., Trautwein W., McDonald T.F. and Pelzer D. J. Physiol. 424:205-228 (1990).

  • Ion channels in prostate cancer
iPLA2-dependent gating of TRPM8 by lysophospholipids.- Vanden Abeele F, Zholos A, Bidaux G, Shuba Y, Thebault S, Beck B, Flourakis M, Panchin Y, Skryma R, Prevarskaya N. J Biol Chem. 2006 Nov 2; [Epub ahead of print]

Prospects for prostate cancer imaging and therapy using high-affinity TRPM8 activators. - Beck B, Bidaux G, Bavencoffe A, Lemonnier L, Thebault S, Shuba Y, Barrit G, Skryma R, Prevarskaya N. Cell Calcium. 2006 Aug 31;[Epub ahead of print].

Differential role of transient receptor potential channels in Ca2+ entry and proliferation of prostate cancer epithelial cells. - Thebault S, Flourakis M, Vanoverberghe K, Vandermoere F, Roudbaraki M, Lehen'kyi V, Slomianny C, Beck B, Mariot P, Bonnal JL, Mauroy B, Shuba Y, Capiod T, Skryma R, Prevarskaya N. Cancer Res. 2006 Feb 15;66(4):2038-47.

Novel role of cold/menthol-sensitive TRPM8 in the activation of store-operated channels in LNCaP human prostate cancer epithelial cells. - Thebault S, Lemonnier L, Bidaux G, Flourakis M, Bavencoffe A, Gordienko D, Roudbaraki M, Delcourt P, Panchin Y,Shuba Y, Skryma R, Prevarskaya N. J Biol Chem. 2005 Nov 25;280(47):39423-35.

Alterations in the regulatory volume decrease (RVD) and swelling-activated Cl- current associated with neuroendocrine differentiation of prostate cancer epithelial cells. - Lemonnier L, Lazarenko R, Shuba Y, Thebault S, Roudbaraki M, Lepage G, Prevarskaya N, Skryma R. Endocr Relat Cancer. 2005 Jun;12(2):335-49.

Bcl-2-dependent modulation of swelling-activated Cl- current and ClC-3 expression in human prostate cancer epithelial cells. - Lemonnier L., Shuba Y., Crepin A., Roudbaraki M., Slomianny C., Mauroy B., Nilius B., Prevarskaya N. and Skryma R. Cancer Res. 64:4841-4848 (2004).

Two types of store-operated Ca2+ channels with different activation modes and molecular origin in LNCaP human prostate cancer epithelial cells. - Vanden Abeele F., Lemonnier L., Thebault S., Lepage G., Parys J., Shuba Y., Skryma R. and Prevarskaya N. J. Biol. Chem. 279:30326-30337 (2004).

2-APB inhibits volume-regulated anion channels independently from intracellular calcium signaling modulation. - Lemonnier L., Prevarskaya N., Mazurier J., Shuba Y. and Skryma R. FEBS Lett. 556:121-126 (2004).

Ca2+ homeostasis and apoptotic resistance of neuroendocrine-differentiated prostate cancer cells. - Vanoverberghe K., Vanden Abeele F., Mariot P., Lepage G., Roudbaraki M., Bonnal J.L., Mauroy B., Shuba Y., Skryma R. and Prevarskaya N. Cell Death Differ. 11:321-330 (2004).

α1-adrenergic receptors activate Ca2+-permeable cationic channels in prostate cancer epithelial cells. - Thebault S., Roudbaraki M., Sydorenko V., Shuba Y., Lemonnier L., Slomianny C., Dewailly E., Bonnal J.L., Mauroy B., Skryma R. and Prevarskaya N. J. Clin. Invest. 111:1691-1701 (2003).

Store-operated Ca2+ channels in prostate cancer epithelial cells: function, regulation, and role in carcinogenesis. - Abeele F.V., Shuba Y., Roudbaraki M., Lemonnier L., Vanoverberghe K., Mariot P., Skryma R. and Prevarskaya N. Cell Calcium. 33:357-373 (2003).

Receptor-coupled, DAG-gated Ca2+-permeable cationic channels in LNCaP human prostate cancer epithelial cells. - Sydorenko V., Shuba Y., Thebault S., Roudbaraki M., Lepage G., Prevarskaya N. and Skryma R. J. Physiol. 548:823-836 (2003).

Store-operated Ca2+ current in prostate cancer epithelial cells: Role of endogenous CaT1 (TRPV6). - Vanden Abeele F., Roudbaraki M., Shuba Y., Skryma R. and Prevarskaya N. J. Biol. Chem. 278:15381-15389 (2003).

Proteolytic modification of swelling-activated Cl- current in LNCaP prostate cancer epithelial cells. - Vitko Y.V., Pogorelaya N.H. Prevarskaya N. and Skryma R. and Shuba Y.M. J. Bioenerg. Biomembr. 34:307-315 (2002).

Direct modulation of volume-regulated anion channels by Ca2+ chelating agents. - Lemonnier L., Vitko Y., Shuba Y.M., Vanden Abeele F., Prevarskaya N. and Skryma R. FEBS Lett. 521:152-156 (2002).

Bcl-2-dependent modulation of Ca2+ homeostasis and store-operated channels in prostate cancer cells. - Vanden Abeele F., Skryma R., Shuba Y., Van Coppenolle F., Slomianny C., Roudbaraki M., Mauroy B., Wuytack F. and Prevarskaya N. Cancer Cell 1:169-179 (2002).

Ca2+ modulation of volume-regulated anion channels: evidence for colocalization with store-operated channels. - Lemonnier L., Prevarskaya N., Shuba Y., Vanden Abeele F., Nilius B., Mazurier J. and Skryma R. FASEB J. 16:222-224 (2002).

Volume-regulated chloride conductance in the LNCaP human prostate cancer cell line. - Shuba Y.M., Prevarskaya N., Lemonnier L., Van Coppenolle F., Kostyuk P.G., Mauroy B. and Skryma R. Am. J. Physiol. Cell Physiol. 279:C1144-1154 (2000).

Store depletion and store-operated Ca2+ current in human prostate cancer cells LNCaP: involvement in apoptosis. - R. Skryma, P. Mariot, X. Bourhis, F. Van Coppenolle F., Shuba Y., F. V. Abeele, G. Legrand, S. Humez, B. Boilly and N. Prevarskaya. J. Physiol. 527:71-83 (2000).

  • Ion channels in glial cells
Tandem-pore domain potassium channels are functionally expressed in retinal (Muller) glial cells. - Skatchkov SN, Eaton MJ, Shuba YM, Kucheryavykh YV, Derst C, Veh RW, Wurm A, Iandiev I, Pannicke T, Bringmann A, Reichenbach A. Glia. 2006 Feb;53(3):266-76.

Tandem-pore K+ channels display an uneven distribution in amphibian retina. - Eaton M.J., Veh R.W., Makarov F., Shuba Y.M., Reichenbach A. and Skatchkov S.N. Neuroreport 15:321-324 (2004).

Chlorotoxin-sensitive Ca2+-activated Cl- channel in type R2 reactive astrocytes from adult rat brain. - Dalton S., Gerzanich V., Chen M., Dong Y., Shuba Y., Simard J.M. Glia 42:325-339 (2003).

  • Technical papers
Glass-funnel technique for the recording of membrane currents and intracellular perfusion of Xenopus oocytes. - Shuba Y.M., Naidenov V.G. and Morad M. Pflügers Arch. 432:562-570 (1996).

A dual-pipette technique that permits rapid internal dialysis and membrane potential measurement in voltage-clamped cardiomyocytes. - Shuba Ya.M., Hesslinger B., Trautwein W., McDonald T.F. and Pelzer D. Pflügers Arch. 415:767-773 (1990).

II. Chapters and Reviews

Calcium signaling and apoptosis resistance of cancer cells. - Prevarskaya N., Skryma R., Shuba Y. - In: Application of apoptosis to cancer treatment. Ed. by M. Sluyser. Dordrecht, Berlin, Heidelberg, New York: Springer, 2005. P. 29-50.

Molecular physiology of K+ channels. - Shuba Y.M. In: Modern problems of cellular and molecular biophysics. Ed. by S.N. Ayrapetyan and A.C.T. North. Yerevan: Noyan Tapan, 2001. P. 343-351.

Multiple types of LVA calcium channel in brain neurons. - Dzhura I., Eremin A., Isaev D., Kostyuk P., Lyubanova O., Naidenov V., Shuba Y. and Tarasenko A. In: Low-Voltage-Activated T-type Calcium Channels. Ed. by R.W. Tsien, J.-P. Clozel and J. Nargeot. Chester: Adis International, 1998. P. 44-52.

Na+-Ca2+ exchanger: from basics to molecular biology. - Shuba Ya.M. Neurophysiology (Kiev) 29:290-296 (1997)

Membrane-delimited stimulation of heart cell calcium current by β-adrenergic signal-transducing Gs protein. - Pelzer S., Shuba Y.M., Birnbaumer L., McDonald T.F. and Pelzer D.J. In: Intracellular regulation of ion channels. Ed. by M. Morad and Z. Agus. Berlin, Heidelberg: Springer-Verlag, 1992. P. 113-127.

Advances in Ca channels studies. - Shuba Ya.M. Neuroscience Facts 2(20):2 (1991).

Direct up-regulating effect of Gs on the whole-cell L-type Ca current in cardiac cells. - Shuba Ya.M., McDonald T.F., Trautwein W., Pelzer S. and Pelzer D. Gen. Physiol. Biophys. 10:105-110 (1991).

Direct and indirect regulation of cardiac L-type calcium channels by β-adrenoreceptor agonists. - Trautwein W., Cavalie A., Allen T.J.A., Shuba Y.M., Pelzer S. and Pelzer D. In: The Biology and Medicine of Signal Transduction. Ed. by Y. Nishizuka et al. New York: Raven Press, 1990. P. 45-50.

Gating and permeation of different types of Ca channels. - Kostyuk P., Akaike N., Osipchuk Yu., Savchenko A. and Shuba Ya. Annals N. Y. Acad. Sci. 560:63-79 (1989).

Kinetic characteristics of different calcium channels in neuronal membrane. - Kostyuk P.G., Shuba Ya.M., Savchenko A.N. and Teslenko V.I. In: The calcium channel: structure, function and implications. Ed. by M. Morad, W. Nayler, S. Kazda and M. Schramm. Berlin, Heidelberg, New York, London, Paris, Tokyo: Springer-Verlag, 1988. P. 424-454.

Teaching:

Undergraduate courses
  • Methods of investigation of biological membranes
  • Biophysics of ion channels

Summary of Research Activities:

I've been involved in the research of membrane ion channels in neuronal, cardiac and other cell types since 1980. I was lucky to contribute to the discovery of the phenomenon of Ca2+-dependent regulation of Ca2+ channels selectivity and permeation (J. Membr .Biol., 1983, 76:83-93), description of neuronal Ca2+ channels diversity (Pflügers Arch., 1988, 411:661-669), characterization of low voltage-activated (LVA) Ca2+ conductance in central neurons (Neurosci., 1996, 70:729-738, Brain Res., 1998, 783:280-285, NeuroReport, 1999, 10:651-652; Pflügers Arch., 2001, 441:832-839), developing of a four-state kinetic model for Ca2+ channel activation (J. Membr. Biol., 1989, 110:29-38, J. Physiol., 1991, 443:25-44), assessment of a direct, Gs protein-mediated pathway in β-adrenergic regulation of cardiac Ca2+ and Cl- channels (J. Physiol., 1990, 424:205-228, Am. J. Physiol., 1990, 259:H264-H267, Am. J. Physiol., 1997, 273:H2539-H2548), characterization of β-adrenergic regulation of cardiac Na+-Ca2+ exchanger (Proc. Natl. Acad. Sci. USA, 1996, 93:5527-5532, J. Biol. Chem., 1998, 273:18819-18825), evaluation of pharmacological and hormonal regulation of cardiac K+ channels (J. Cardiovasc. Pharm., 1997, 30:42-45, J. Pharm. Exp. Ther., 1997, 281:233-244, Biochem. Pharm., 2001, 62:41-49), characterization of membrane conductances in prostate cancer epithelial cells (Am. J. Physiol., 2000, 279:C1144-1154, J. Physiol. 2000, 527:71-83, FASEB J., 2002, 16:222-224, Cancer Cell, 2002, 1:169-179, J. Bioenerg. Biomembr., 2002, 34:307-315).

My research career is closely associated with Bogomoletz Institute of Physiology, National Academy of Sciences of Ukrainian, Kiev, Ukraine, where I began to work in 1980 as Ph.D. student under the supervision of Prof. P. Kostyuk, received my two scientific degrees (Ph.D. in 1983 and D.Sc. in 1991), and continue to work up to now as Leading Scientist, research group leader and deputy head of the Department of General Physiology of Nervous System. In recent years my research was primarily focused on investigation of the diversity of low voltage-activated Ca2+ channels in central, particularly thalamic neurons, and on characterization of pharmacology and regulation by sex steroid hormones of HERG K+ channel. During the period of 1993-96 I worked as visiting assistant professor at Georgetown University (Washington, DC, USA) in close association with Dr. M. Morad, where I studied the mechanisms of β-adrenergic regulation of cardiac Na+-Ca2+ exchanger and pharmacology of cardiac K+ channels. Since 1998 I also hold invited professorship at the University of Lille1 (France) for 3 to 4 months per academic year, where with my colleagues, Prof. N. Prevarskaya and Prof. R. Skryma, I was involved in the discovery and characterization of volume-regulated Cl- conductance and store-operated Ca2+ entry in the prostate cancer epithelial cells. These topics continue to be among our first priorities. In my experimental work I like to improve things, develop new approaches and introduce various innovations. I proposed two new experimental techniques - double pipette technique for rapid dialysis of small cells (Pflüg. Arch., 1988, 415:767-773) and perfusion technique for Xenopus oocytes (Pflüg. Arch., 1996, 432:562-570). I was recipient of a number of research grants from various International granting agencies. I teach two courses: "Methods of investigation of biological membranes" and "Biophysics of ion channels" to undergraduate students from Kiev Shevchenko National University and some other educational institutions.